Fast imaging of DNA motion reveals distinct sub-diffusion regimes at the site of DNA damage

نویسندگان

  • Judith Miné-Hattab
  • Vincent Recamier
  • Ignacio Izeddin
  • Rodney Rothstein
  • Xavier Darzacq
چکیده

1 Institut de Biologie de l’Ecole Normale Superieure (iBENS), Paris 75005, France 2 Department of Genetics & Development, Columbia University Medical Center, New York, NY 10032, USA 3 Nuclear Dynamics, CNRS UMR 3664, Institut Curie, Paris 75005, France 4 Institut Langevin, ESPCI Paris Tech, CNRS UMR 7587, PSL Research University, 75005 Paris, France 5 Division of Genetics, Genomics & Development, Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA 6 Laboratory Imaging, s. r. o. Za Drahou 171/17 102 00 Praha 10, Czech Republic

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Multi-scale tracking reveals scale-dependent chromatin dynamics after DNA damage

The dynamic organization of genes inside the nucleus is an important determinant for their function. Using fast DNA tracking microscopy in S. cerevisiae cells and improved analysis of mean square displacements, we quantified DNA motion at time scales ranging from 10 milliseconds to minute and found that following DNA damage, DNA exhibits distinct sub-diffusive regimes. In response to double-str...

متن کامل

تحلیل رفتار DNA در گذر از ریز ساختارها بر اساس معادله فوکر-پلانک و مدل سد آنتروپی

We considered the motion of DNA molecules through a hexagonal array under uniform electric fields as a Fokker-Planck process which is affected by the entropic barriers and we have simulated this motion by computer. We solved the Fokker-Planck equation with numerical simulation of the Brownian dynamics by the Euler method. For different DNA molecules, under different physical conditions, the mea...

متن کامل

DNA Damages on Blood Cells After Cardiac Magnetic Resonance Imaging

Introduction: Along with the increased use of cardiac imaging at clinics there is increased attention to the potential risks related to the methods used like magnetic resonance (MR) and it cannot be ruled out that MR can alter DNA structure. The aim of this review is to assess the impact of routine cardiac magnetic resonance (CMR) scanning on DNA damages in human T lymphocytes....

متن کامل

DNA Damage in Leukocytes from Fanconi Anemia (FA) Patients and Heterozygotes Induced by Mitomycin C and Ionizing Radiation as Assessed by the Comet and Comet-FISH Assay

Background: Lymphocytes of Fanconi anemia (FA) show an increased sensitivity to the alkylating agents such as mitomycin C (MMC), but their responses to gamma-irradiation is controversial. The extent of DNA damage in leukocytes of FA patients following irradiation and MMC treatment was studied at cellular and single chromosome level. Methods: DNA damage induced by gamma-rays and MMC was measure...

متن کامل

Studies on electron beam induced DNA damage and repair kinetics in lymphocytes by alkaline comet assay

Background: Exposure to ionizing radiation is known to induce oxidative stress followed by damage to critical biomolecules like lipids, proteins and DNA through radiolysis of cellular water. Since radiation has been widely used as an important tool in therapy of cancer, the detailed investigation regarding the DNA damage and repair kinetics would help to predict the radiation sensitivity of cel...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

عنوان ژورنال:

دوره   شماره 

صفحات  -

تاریخ انتشار 2015